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Simian Practicalist

Study: Pfizer “Vaccines” Contain DNA Contaminants 3 to 4 Times Maximal Acceptable Concentration

A German study by U. Kammerer et al titled “BioNTech RNA-Based COVID-19 Injections Contain Large Amounts Of Residual DNA Including An SV40 Promoter/Enhancer Sequence” posted on 3 December 2024 has found that Pfizer “vaccines” contain three to four times the maximal acceptable concentration of DNA contaminants set by regulatory bodies.


The talk of COVID-19 injections containing contaminants has been circulating for quite some time, including DNA contaminants.

This possibility emerged on the scene in February 2023, when McKernan and colleagues announced the discovery of large amounts of both spike-coding DNA and residual plasmid-DNA derived from the expression vector system in BioNTech/Pfizer and Moderna vaccine lots. The bulk was represented by fragmented, linearized DNA, but also intact plasmids being able to successfully transfect E. coli cells. … Incomprehensibly, plasmids from BioNTech/Pfizer, but not from Moderna, do not only contain the bacterial T7 promoter system, but also the mammalian Simian Virus 40 (SV40) promoter/enhancer sequence. This gives cause for concern, as already in 1999, Dean and colleagues demonstrated that nuclear entry of plasmid-DNA, especially in non-dividing cells, requires a 72 bp sequence of the SV40 promoter/enhancer. Of note, neither the promoter, nor the origin of replication are needed for nuclear localization of plasmid-DNA. Meanwhile, the results of the McKernan team have been confirmed and extended. Recently, König and Kirchner published data on large amounts of residual DNA within several BNT162b2 lots.

The experiments were conducted on three monovalent lots (FD7958, FE6975, EX8679) and one bivalent lot (HD9869). Measurements were made over 7 days to cover spike protein expression over time.


Whilst the researchers found that the amount of RNA fits with the manufacturer’s claim of 30 µg per clinical dose, DNA concentration

…in the vials analyzed in this study ranged from 32.7 to 43.4 ng per clinical dose after eliminating RNA via RNase digestion. This far exceeds the maximal upper limit of 10 ng per clinical dose, which the WHO has declared to be tolerable in injectable biologicals.

It was suspected even before the rollout that the production of spike proteins may last more than “a couple of days”.

After one week, even more spike proteins are present in the cells than after 24 hours, although the highest modRNA level is expected after 24 hours, before it is degraded. The amount of spike proteins produced varied between lots, but the progression over time was identical for all, with a peak on day 5 after transfection.
Figure 1C: Quantification of intracellular spike protein (SP) concentrations over time measured by ELISA. Figure 1D: Quantification of secreted spike protein (SP) levels over time measured by ELISA.
Figure 1C: Quantification of intracellular spike protein (SP) concentrations over time measured by ELISA. Figure 1D: Quantification of secreted spike protein (SP) levels over time measured by ELISA.

Back to the presence of a DNA sequence of the SV40 promoter/enhancer:

This sequence was not declared in the plasmid map that BioNTech/Pfizer submitted in the approval procedure. This finding is very surprising and raises the legitimate question: Why did BioNTech/Pfizer apply this totally unnecessary but highly dangerous element in their plasmids and use it as a template for the production of modRNA? In our opinion, BioNTech/Pfizer must be held accountable for incorporating this highly dangerous element in their plasmids.

For a scientific/academic paper, this is as if the writer has no manners and is declaring someone needs to be executed by hanging.


The researchers conclude by listing four dangers of “RNA biologicals”:

First, modRNA encoding any foreign protein will trigger detrimental autoimmune reactions. Second, the lipid nanoparticles are themselves highly toxic. Third, residual plasmid-DNA and reverse transcribed mRNA will genetically modify cells. Fourth, replacement of uridine in natural mRNA by N1-methyl-pseudouridine in synthetic modRNA causes +1 ribosomal frameshifting resulting in haphazard production of utterly alien proteins.

In other words, they “call for an immediate halt of all RNA-based biologicals until these concerns are scientifically addressed and convincingly dispelled”.


Table 4: CRNA and DNA levels (following Triton-X-100 treatment) in the indicated vials of BioNTech lots before and after RNase A treatment.
Table 4: CRNA and DNA levels (following Triton-X-100 treatment) in the indicated vials of BioNTech lots before and after RNase A treatment.
 

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